Views: 11 Author: Site Editor Publish Time: 2022-01-12 Origin: Site
The structure of potassium 4-methoxysalicylate (4MSK) is similar to salicylic acid. It is a new type of medicinal whitening ingredient. It is used in cosmetics and has a whitening effect. It is an effective ingredient in cosmetics. It has the functions of improving skin tone balance, relieving excessive pigmentation, correcting atypical epidermal proliferation, promoting the discharge of melanin, preventing freckles and spots, thereby creating smooth skin.
After culturing mouse melanoma cells (B16) with potassium 4-methoxysalicylate and without potassium 4-methoxysalicylate for three days respectively, the number of cells and the melanin under a certain number of cells were detected quantity. The ratio of the number of cells and the amount of melanin at the time of addition compared with that when potassium 4-methoxysalicylate is not added is obtained. In this way, the effect of potassium 4-methoxysalicylate on melanin production is analyzed. effect.
The melanin metabolism research report performed the observation of the differentiation expression of the three-dimensional cultured skin model on the plate, mainly observing the level of "pocket protein" in the granular layer, and found that the skin added with 4-methoxysalicylate (4MSK) was irradiated by ultraviolet rays. Model, significantly inhibited the decrease in the expression of pocket protein by ultraviolet rays. The observation from the in vitro experimental model, 4-methoxy potassium salicylate (4MSK) and salicylic acid have a certain similarity in function, which can promote the smooth metabolism of melanin.
The key to studying whitening ingredients is to analyze the actual production of melanin and the inhibition of tyrosinase. In short, potassium 4-methoxysalicylate (4MSK) can inhibit melanin production and tyrosinase activity in a certain concentration environment. The concentration dependence of the former is 310-4 and 10-3mol/L, and the concentration dependence of the latter is effective in the range of 510-4~310-3mol/L.
For the evaluation of the tyrosinase activity of the non-cellular system, L-DOPA was used as the substrate, and the amount of dopa pigment produced by its metabolism was detected by the test method of 475mn absorbance. The inhibition rate of tyrosinase activity of potassium 4-methoxysalicylate is that potassium 4-methoxysalicylate solution and solvent are added to the above reaction system, and the mixture is placed at room temperature for 90 seconds , Obtained the ratio of the test value when adding 4-methoxy potassium salicylate solution compared with the vehicle group.
After irradiating the three-dimensional skin model with ultraviolet rays, immediately add a potassium 4-methoxysalicylate solution to the surface of the skin model, and after incubating for 30 minutes, remove the potassium 4-methoxysalicylate and continue the culture for 1 day. Instead of potassium 4-methoxysalicylate solution, use a solvent to control it. For the skin model sampled after culture, immunohistochemical study was carried out with anti-Loring protein antibody staining, and the real-time R-TPcR (reverse transcription polymerase chain reaction) was used to quantify the expression of loric protein mRNA.
In 4 abdomens of 12 healthy adult male volunteers, each tester was irradiated with an artificial ultraviolet light source with UV gas and UVB wavelength ultraviolet light once a day, equivalent to IMED, for 3 consecutive days. A total of 4 creams mixed with 1%, 3%, and 5% potassium 4-methoxysalicylate and base creams were used as test samples, 3 times a day from the day when the ultraviolet rays were irradiated, continuous Apply for 7 days. When evaluating the degree of pigmentation, use a color tester to measure the skin color (L* value) before and after the application of the sample three times, calculate the average value, and obtain the change before and after the irradiation (△L*), and compare different concentrations The area where the potassium 4-methoxysalicylate cream is applied and the area where the base cream is applied.
The report shows that in vivo experiments under certain sunlight conditions, 1% potassium 4-methoxysalicylate (4MSK) product has the effect of reducing PIH, and the more the concentration increases, the more significant the decrease.